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阿托品对大鼠大脑皮质切片中乙酰胆碱释放和合成的刺激作用。

Stimulation by atropine of acetylcholine release and synthesis in cortical slices from rat brain.

作者信息

Molenaar P C, Polak R L

出版信息

Br J Pharmacol. 1970 Nov;40(3):406-17. doi: 10.1111/j.1476-5381.1970.tb10622.x.

DOI:10.1111/j.1476-5381.1970.tb10622.x
PMID:5497792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1703171/
Abstract
  1. Cortical slices from rat brain were incubated in media containing the irreversible cholinesterase inhibitor soman and a high KCl concentration, and the release and synthesis of acetylcholine (ACh) were determined.2. Atropine enhanced the release and synthesis of ACh.3. Tetrodotoxin, a substance which blocks nervous conduction, did not influence the release and synthesis of ACh, in the absence or in the presence of atropine. Therefore the nerve endings are probably the site at which atropine acts when stimulating the release and synthesis of ACh.4. Pretreatment of the slices with botulinum type A toxin partially blocked the release and synthesis of ACh and reduced the extra amounts of ACh released and synthesized under the influence of atropine.5. Lowering the calcium or raising the magnesium concentration in the incubation medium reduced the release and synthesis of ACh and their enhancement by atropine.6. Physostigmine decreased the total extractable ACh content of the slices during incubation in a 25 mM KCl containing medium. This decrease was nearly prevented when the release and synthesis of ACh were inhibited by omission of the calcium ions from the medium, but was enhanced by atropine.7. The observations made with pretreatment by botulinum type A toxin, with changes in the calcium and magnesium concentration as well as with physostigmine, all support the theory that it is primarily the release of ACh which is enhanced by atropine and that its stimulating action on the synthesis results from the increased release.
摘要

  1. 将大鼠脑皮质切片置于含有不可逆胆碱酯酶抑制剂梭曼和高浓度氯化钾的培养基中孵育，然后测定乙酰胆碱（ACh）的释放和合成。

  2. 阿托品增强了ACh的释放和合成。

  3. 河豚毒素是一种阻断神经传导的物质，在不存在或存在阿托品的情况下，均不影响ACh的释放和合成。因此，神经末梢可能是阿托品刺激ACh释放和合成时的作用位点。

  4. 用A型肉毒杆菌毒素预处理切片可部分阻断ACh的释放和合成，并减少在阿托品影响下释放和合成的额外ACh量。

  5. 降低孵育培养基中的钙浓度或提高镁浓度会降低ACh的释放和合成以及阿托品对它们的增强作用。

  6. 在含有25 mM氯化钾的培养基中孵育期间，毒扁豆碱降低了切片中可提取的总ACh含量。当培养基中省略钙离子抑制ACh的释放和合成时，这种降低几乎被阻止，但阿托品会增强这种降低。

  7. 用A型肉毒杆菌毒素预处理、钙和镁浓度的变化以及毒扁豆碱的观察结果均支持以下理论：阿托品主要增强的是ACh的释放，其对合成的刺激作用源于释放的增加。

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Stimulation by atropine of acetylcholine release and synthesis in cortical slices from rat brain.阿托品对大鼠大脑皮质切片中乙酰胆碱释放和合成的刺激作用。
Br J Pharmacol. 1970 Nov;40(3):406-17. doi: 10.1111/j.1476-5381.1970.tb10622.x.
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本文引用的文献

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The spontaneous and evoked release of acetylcholine from the cerebral cortex.乙酰胆碱从大脑皮层的自发释放和诱发释放。
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Acetylcholine metabolism in the central nervous system: The effects of potassium and other cations on acetylcholine liberation.中枢神经系统中的乙酰胆碱代谢：钾及其他阳离子对乙酰胆碱释放的影响。
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THE EFFECT OF TERTIARY AND QUATERNARY ATROPINE ON CORTICAL ACETYLCHOLINE OUTPUT AND ON THE ELECTROENCEPHALOGRAM IN CATS.叔胺和季铵阿托品对猫皮质乙酰胆碱释放及脑电图的影响
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ON THE BIOSYNTHESIS OF ACETYL COENZYME A IN THE BRAIN. I. THE ENZYMIC FORMATION OF ACETYL COENZYME A FROM ACETATE, ADENOSINE TRIPHOSPHATE AND COENZYME A.论大脑中乙酰辅酶A的生物合成。I. 由乙酸、三磷酸腺苷和辅酶A酶促形成乙酰辅酶A
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THE INFLUENCE OF CENTRALLY ACTING CHOLINOLYTIC DRUGS ON BRAIN ACETYLCHOLINE LEVELS.中枢性抗胆碱能药物对脑乙酰胆碱水平的影响。
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SYNTHESIS AND STORAGE OF ACETYLCHOLINE IN NERVOUS TISSUE.神经组织中乙酰胆碱的合成与储存
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The effects of presynaptic polarization on the spontaneous activity at the mammalian neuromuscular junction.突触前极化对哺乳动物神经肌肉接头处自发活动的影响。
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An intracellular study of the action of repetitive nerve volleys and of botulinum toxin on miniature end-plate potentials.关于重复神经冲动和肉毒杆菌毒素对微小终板电位作用的细胞内研究。
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