阿托品对大鼠大脑皮层体外释放乙酰胆碱的刺激作用。
Stimulating action of atropine on the release of acetylcholine by rat cerebral cortex in vitro.
作者信息
Polak R L
出版信息
Br J Pharmacol. 1971 Apr;41(4):600-6. doi: 10.1111/j.1476-5381.1971.tb07068.x.
Abstract
- In cortical slices from rat brain incubated in a medium containing the irreversible cholinesterase inhibitor, soman (0.005 mM) and a high concentration of KCl (25 mM), atropine exerts a stimulating action on the release of acetylcholine (ACh).2. Two possible explanations for this action were examined. Atropine might expel ACh from the nerve endings by occupying its storage sites or it might prevent an inhibitory action of the released ACh on its further release by occupying muscarinic receptors at the presynaptic endings; its action would then be a kind of ;disinhibition.'3. The stimulating action of atropine on ACh release persisted during prolonged incubation (up to 3.5 h) provided choline was added to the medium. This finding would be difficult to explain if atropine acted by expelling ACh from its storage sites.4. The stimulating action of atropine on ACh release was inhibited by oxotremorine and by methacholine, added to the medium in high concentrations. This finding is readily explained if atropine acts by ;disinhibition.'
摘要
在含有不可逆胆碱酯酶抑制剂梭曼（0.005 mM）和高浓度氯化钾（25 mM）的培养基中孵育的大鼠脑皮质切片中，阿托品对乙酰胆碱（ACh）的释放具有刺激作用。
研究了对此作用的两种可能解释。阿托品可能通过占据其储存位点将ACh从神经末梢排出，或者它可能通过占据突触前末梢的毒蕈碱受体来阻止释放的ACh对其进一步释放的抑制作用；其作用将是一种“去抑制”。
如果向培养基中添加胆碱，阿托品对ACh释放的刺激作用在长时间孵育（长达3.5小时）期间持续存在。如果阿托品通过将ACh从其储存位点排出而起作用，这一发现将难以解释。
高浓度添加到培养基中的氧化震颤素和乙酰甲胆碱可抑制阿托品对ACh释放的刺激作用。如果阿托品通过“去抑制”起作用，这一发现很容易解释。
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本文引用的文献
1
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Am J Physiol. 1950 Aug 1;162(2):469-74. doi: 10.1152/ajplegacy.1950.162.2.469.
2
The influence of atropine on the release and uptake of acetylcholine by the isolated cerebral cortex of the rat.阿托品对大鼠离体大脑皮层乙酰胆碱释放和摄取的影响。
Biochem Pharmacol. 1966 Jul;15(7):989-92. doi: 10.1016/0006-2952(66)90176-6.
3
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