Wilhelm A R, Gerone P J
Appl Microbiol. 1970 Oct;20(4):612-5. doi: 10.1128/am.20.4.612-615.1970.
The growth of Rio Bravo virus (RBV) in eight cell culture systems was studied. Highest yields of virus were produced in BHK-21 (C13), L, and Vero cell lines, but L cells were resistant to low doses of virus. LLC-MK(2), HeLa, and human embryo skin cells produced moderate amounts of virus, but FL amnion and primary chick embryo fibroblasts supported little virus growth. Virus was rapidly inactivated by exposure to pH values below 7.0. Single-cycle growth in BHK-21, L, and LLC-MK(2) cell monolayers was characterized by a latent period of about 12 hr followed by rapid virus production that peaked at 36 to 48 hr. Vero cell cultures can remain chronically infected with RBV for more than 100 days. Such cultures show evidence of cell destruction, and their supernatant fluids contain virus at 10(4) to 10(5) log(10) per ml.
对里约布拉沃病毒（RBV）在八种细胞培养系统中的生长情况进行了研究。在BHK-21（C13）、L和Vero细胞系中产生的病毒产量最高，但L细胞对低剂量病毒具有抗性。LLC-MK(2)、HeLa和人胚胎皮肤细胞产生适量的病毒，但FL羊膜和原代鸡胚成纤维细胞几乎不支持病毒生长。病毒暴露于pH值低于7.0时会迅速失活。BHK-21、L和LLC-MK(2)细胞单层中的单循环生长特点是潜伏期约为12小时，随后病毒快速产生，在36至48小时达到峰值。Vero细胞培养物可被RBV持续感染超过100天。此类培养物显示出细胞破坏的迹象，其上清液中每毫升含有10(4)至10(5) log(10)的病毒。
