Ajello C A
J Med Virol. 1979;3(4):301-6. doi: 10.1002/jmv.1890030408.
The susceptibility and sensitivity of Aedes albopictus cell cultures to five different primary and four different low-passage arboviruses were tested. Yellow fever, West Nile, Ilesha, eastern equine encephalitis, and Flanders viruses replicated in A albopictus tissue cultures. Replication was determined by the ability of selected tissue culture fluids to infect suckling mice, and by recovery from tissue culture fluid of progressively increasing amounts of complement-fixing (CF) antigen with time. Virus persistence was demonstrated with Nodamura, western equine encephalitis, and Mayaro viruses, but multiplication was not proven; neither persistence nor multiplication was demonstrated with a Kemerovo group virus. When yellow fever and Ilesha viruses were simultaneously inoculated into A albopictus culture, CF antigen for each was consistently detected. In a more detailed comparative study of field specimens, 12 unpassaged strains of yellow fever virus were tested for infectivity in A albopictus tissue culture, Vero cells, and baby mice. Higher titers of virus were detected (0.8--2.3 log ID50 per ml) in Vero cell culture than in A albopictus tissue culture or baby mouse systems. These results suggest the feasibility of using A albopictus cells in association with the primary isolation of arboviruses.
测试了白纹伊蚊细胞培养物对五种不同的原始病毒和四种不同的低代虫媒病毒的易感性和敏感性。黄热病病毒、西尼罗河病毒、伊莱沙病毒、东部马脑炎病毒和佛兰德斯病毒在白纹伊蚊组织培养物中复制。通过所选组织培养液感染乳鼠的能力以及随着时间的推移从组织培养液中逐渐回收越来越多的补体结合（CF）抗原，来确定病毒的复制情况。诺达木拉病毒、西部马脑炎病毒和马亚罗病毒表现出病毒持续性，但未证实有增殖；克麦罗沃组病毒既未表现出持续性也未表现出增殖。当黄热病病毒和伊莱沙病毒同时接种到白纹伊蚊培养物中时，始终能检测到每种病毒的CF抗原。在一项关于野外标本的更详细的比较研究中，测试了12株未传代的黄热病病毒在白纹伊蚊组织培养物、非洲绿猴肾细胞（Vero细胞）和乳鼠中的感染性。在Vero细胞培养物中检测到的病毒滴度（每毫升0.8 - 2.3对数ID50）高于白纹伊蚊组织培养物或乳鼠系统。这些结果表明将白纹伊蚊细胞用于虫媒病毒的初次分离是可行的。
