Bovina C, Tolomelli B, Formiggini G, Marchetti M
Int J Vitam Nutr Res. 1979;49(4):396-9.
The distribution in liver of pteroylpolyglutamates and urinary excretion of folate metabolites was studied in folic acid deficient rats after injection of the vitamin, in order to evaluate the effect of folic acid deficiency on the conversion of the vitamin into actual coenzymic forms. Any significant difference neither in total activity nor in pteroylpolyglutamates of liver does not appear between deficient supplemented and unsupplemented animals. Also the urinary amounts of reduced metabolites in deficient rats do not increase after injection of folic acid. Therefore the inability of deficient rat liver to synthesize polyglutamates may be ascribed to the lower availability of tetrahydrofolates as they are the preferred substrates of pteroylpolyglutamate synthetase.
为了评估叶酸缺乏对维生素转化为实际辅酶形式的影响，研究了注射维生素后叶酸缺乏大鼠肝脏中蝶酰多谷氨酸的分布和叶酸代谢产物的尿排泄情况。在缺乏叶酸但补充和未补充叶酸的动物之间，肝脏的总活性和蝶酰多谷氨酸均未出现任何显著差异。此外，注射叶酸后，缺乏叶酸大鼠尿液中还原代谢产物的量也没有增加。因此，缺乏叶酸的大鼠肝脏无法合成多谷氨酸，可能是由于四氢叶酸的可用性较低，因为它们是蝶酰多谷氨酸合成酶的首选底物。
