Formiggini G, Bovina C, Tolomelli B, Marchetti M
Acta Vitaminol Enzymol. 1983;5(4):225-8.
In order to evaluate the effect of folate deficiency on pteroylpolyglutamate synthesis in rat liver, the hepatic and urinary content of reduced folates, the distribution of pteroylpolyglutamates and the incorporation of i.p. injected 3H folic acid in polyglutamates have been studied. In deficient rats the hepatic content of reduced folates and the urinary amounts of reduced metabolites are lower and do not increase after vitamin treatment. Longer chain pteroylpolyglutamates are severely depleted and their content is not significantly modified by folic acid treatment. The percentage of labelled folic acid incorporated into pteroylglutamates is markedly decreased. The inability of deficient rat liver to synthesize polyglutamates might be ascribed to the decrease of pteroylpolyglutamate synthetase activity, and/or to the lower availability of tetrahydrofolates, the preferred substrates for the enzyme.
为了评估叶酸缺乏对大鼠肝脏中蝶酰多聚谷氨酸合成的影响，研究了还原型叶酸的肝脏和尿液含量、蝶酰多聚谷氨酸的分布以及腹腔注射的3H叶酸在多聚谷氨酸中的掺入情况。在缺乏叶酸的大鼠中，还原型叶酸的肝脏含量和还原型代谢产物的尿液量较低，维生素治疗后也不会增加。长链蝶酰多聚谷氨酸严重缺乏，叶酸治疗后其含量没有明显改变。掺入蝶酰谷氨酸的标记叶酸百分比明显降低。缺乏叶酸的大鼠肝脏无法合成多聚谷氨酸，可能归因于蝶酰多聚谷氨酸合成酶活性的降低，和/或该酶的首选底物四氢叶酸的可用性降低。
