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一种不溶性链霉蛋白酶衍生物的制备、性质及底物排斥效应

Preparation, properties and substrate-exclusion effects of an insoluble pronase derivative.

作者信息

Cresswell P, Sanderson A R

出版信息

Biochem J. 1970 Sep;119(3):447-51. doi: 10.1042/bj1190447.

DOI:10.1042/bj1190447
PMID:5500304
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1179373/
Abstract
  1. A diazotized co-polymer of leucine and p-aminophenylalanine was used to prepare insoluble pronase. 2. The product was similar to the native enzyme in pH optimum, temperature optimum and broad specificity. 3. Exclusion effects were observed that appear related to the molecular weight of the substrate being hydrolysed. 4. The effects are explained on the basis of impedance of substrate access to the catalytic site by the supporting solid matrix.
摘要
  1. 亮氨酸和对氨基苯丙氨酸的重氮化共聚物被用于制备不溶性链霉蛋白酶。2. 该产物在最适pH、最适温度和广泛特异性方面与天然酶相似。3. 观察到了排除效应，其似乎与被水解底物的分子量有关。4. 这些效应是基于支持性固体基质对底物进入催化位点的阻碍来解释的。