Hashimoto K, Kanzaki T
Acta Derm Venereol. 1975;55(6):413-30.
The normal human skin surface was studied with a combination of scanning electron microscope, surface replication method, and transmission electron microscope. All specimens were prepared with the critical point drying (CPD) method. Horny cells were found to stack up in columnar fashion with their peripheries overlapping. The surface of a non-nucleated horny cell, therefore, showed a dome-shaped elevation in the center, transmitting the nuclear bulge of the underlying granular cells; parallel double lines at the periphery representing its true cell border and depressed, step-like imprint of the cell border of the cell which once overlapped; and on high magnification the true surface of the separated desmosomes. The surfaces of the horny cells showed many bizarre marks but were relatively smooth. The underside of the surface horny cells, however, bore villi except in areas overlapping the periphery of the underlying cell. Tissue cultured keratinocytes also stacked up when the keratinized and the cell periphery also overlapped. However, the overlapped cell border did not leave the imprint on the underlying cell, probably because of the lack of pressure from above.
采用扫描电子显微镜、表面复型法和透射电子显微镜相结合的方法对正常人体皮肤表面进行了研究。所有标本均采用临界点干燥（CPD）法制备。发现角质形成细胞呈柱状堆积，其周边相互重叠。因此，无核角质形成细胞的表面在中央呈现出圆顶状隆起，传递出下方颗粒细胞的核凸起；周边的平行双线代表其真正的细胞边界以及曾经重叠的细胞的细胞边界的凹陷、阶梯状印记；在高倍镜下可看到分离的桥粒的真实表面。角质形成细胞的表面有许多奇异的痕迹，但相对光滑。然而，表面角质形成细胞的底面除了与下方细胞周边重叠的区域外，都有绒毛。组织培养的角质形成细胞在角质化时也会堆积，细胞周边也会重叠。然而，重叠的细胞边界没有在下方细胞上留下印记，可能是因为缺乏来自上方的压力。
