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神经刺激和半胆碱对哺乳动物神经肌肉接头处突触小泡的影响。

The effects of nerve stimulation and hemicholinium on synaptic vesicles at the mammalian euromuscular junction.

作者信息

Jones S F, Kwanbunbumpen S

出版信息

J Physiol. 1970 Mar;207(1):31-50. doi: 10.1113/jphysiol.1970.sp009046.

DOI:10.1113/jphysiol.1970.sp009046
PMID:5503879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1348690/
Abstract
  1. Electron micrographs of nerve terminals in rat phrenic nerve-diaphragm preparations have been studied. This has been done before and after prolonged nerve stimulation. The effectiveness of nerve stimulation has been monitored by intracellular micro-electrode recordings from the muscle cells.2. Characteristic changes in the form and distribution of the nerve terminal mitochondria were noted after nerve stimulation.3. Synaptic vesicle numbers in the region of nerve terminal less than 1800 A from the synaptic cleft were significantly greater in tissue taken 2 and 3 min after nerve stimulation, than in unstimulated preparations.4. The long and short diameters of the synaptic vesicle profiles less than 1800 A from the synaptic cleft were measured. Analysis of the distribution of the diameters indicated synaptic vesicles to be basically spherical structures. Estimates of synaptic vesicle volume were made from the measurements. Synaptic vesicle volume was significantly reduced in tissue taken 2 and 4 min following nerve stimulation.5. If hemicholinium, a compound which inhibits acetylcholine synthesis, was present during the period of nerve stimulation, much greater reductions in synaptic vesicle volume occurred. Synaptic vesicle numbers in the region of nerve terminal less than 1800 A from the synaptic cleft were also reduced, compared with unstimulated control preparations.6. These results are regarded as support for the hypothesis that the synaptic vesicles in nerve terminals at the mammalian neuromuscular junction represent stores of the transmitter substance, acetylcholine.
摘要

  1. 对大鼠膈神经 - 膈肌标本中神经末梢的电子显微镜照片进行了研究。这一研究在长时间神经刺激前后均已开展。神经刺激的效果通过肌肉细胞的细胞内微电极记录进行监测。

  2. 神经刺激后，神经末梢线粒体的形态和分布出现了特征性变化。

  3. 神经刺激后2分钟和3分钟采集的组织中，距离突触间隙小于1800埃的神经末梢区域内的突触小泡数量，显著多于未受刺激的标本。

  4. 测量了距离突触间隙小于1800埃的突触小泡轮廓的长径和短径。直径分布分析表明突触小泡基本为球形结构。根据测量结果对突触小泡体积进行了估算。神经刺激后2分钟和4分钟采集的组织中，突触小泡体积显著减小。

  5. 如果在神经刺激期间存在抑制乙酰胆碱合成的化合物半胆碱，突触小泡体积的减小幅度会大得多。与未受刺激的对照标本相比，距离突触间隙小于1800埃的神经末梢区域内的突触小泡数量也减少了。

  6. 这些结果被视为支持以下假说：哺乳动物神经肌肉接头处神经末梢中的突触小泡代表了神经递质乙酰胆碱的储存库。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/f11692c7d034/jphysiol01056-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/08417efecbc5/jphysiol01056-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/79b24d639c2d/jphysiol01056-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/82a7319f7c62/jphysiol01056-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/501ce108ae7a/jphysiol01056-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/d8a91ce7e6ab/jphysiol01056-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/00d344775a05/jphysiol01056-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/f11692c7d034/jphysiol01056-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/08417efecbc5/jphysiol01056-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/79b24d639c2d/jphysiol01056-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/82a7319f7c62/jphysiol01056-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/501ce108ae7a/jphysiol01056-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/d8a91ce7e6ab/jphysiol01056-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/00d344775a05/jphysiol01056-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d16/1348690/f11692c7d034/jphysiol01056-0083-b.jpg

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