人低密度脂蛋白与大鼠红细胞“血影”之间未酯化胆固醇的交换
The exchange of unesterified cholesterol between human low-density lipoproteins and rat erythrocyte 'ghosts'.
作者信息
Bruckdorfer K R, Green C
出版信息
Biochem J. 1967 Jul;104(1):270-7. doi: 10.1042/bj1040270.
Abstract
- The exchange of unesterified cholesterol molecules between rat erythrocyte ;ghosts' and human low-density lipoproteins has been studied under a number of different experimental conditions. 2. The process is pH-dependent, the rate being minimal at about pH5. 3. Cholesterol exchange does not vary greatly with temperature, the rate at 50 degrees being less than twice that at 2 degrees . 4. Large variations in the ionic strength or Ca(2+) concentration of the medium have little effect, but the exchange rate is greatly increased in the presence of a wide range of chemical compounds, e.g. urea, alcohols, acetone, dimethyl sulphoxide and tetra-alkyl-ammonium salts. 5. Acetone and dimethyl sulphoxide have a much greater effect at 37 degrees than at 8-10 degrees . 6. It is proposed that hydrophobic bonding is of great importance in maintaining the structure of ;ghosts' and lipoproteins. 7. The results are discussed in relation to current theories of membrane and lipoprotein structure.
摘要
- 在多种不同实验条件下，对大鼠红细胞“血影”与人类低密度脂蛋白之间未酯化胆固醇分子的交换进行了研究。2. 该过程依赖于pH值，在约pH5时速率最小。3. 胆固醇交换随温度变化不大，50摄氏度时的速率不到2摄氏度时的两倍。4. 介质的离子强度或Ca(2+)浓度有很大变化时影响不大，但在多种化合物（如尿素、醇类、丙酮、二甲基亚砜和四烷基铵盐）存在时，交换速率会大大增加。5. 丙酮和二甲基亚砜在37摄氏度时的作用比在8 - 10摄氏度时大得多。6. 有人提出疏水键在维持“血影”和脂蛋白的结构中非常重要。7. 根据当前关于膜和脂蛋白结构的理论对结果进行了讨论。
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