从牛红细胞膜上去除血型决定簇。2. 用丁醇和吡啶降解血影。

Removal of blood group determinants from bovine erythrocyte membranes. 2. Degradation of ghosts by butanol and pyridine.

作者信息

Zink G L, Hines H C

出版信息

Anim Blood Groups Biochem Genet. 1975;6(4):235-43. doi: 10.1111/j.1365-2052.1975.tb01368.x.

DOI:10.1111/j.1365-2052.1975.tb01368.x

Abstract

Ghosts produced from erythrocytes collected from six different cattle were degraded with butanol and pyridine. Of a total of 38 different antigenic determinants available for investigation among the six cows, F, V, J and L were the only specificities detected in the subfractions resulting from either method of degradation. After butanol degradation V, J and L antigens were found in the soluble protein fraction, while F was found in the insoluble protein. Pyridine digestion resulted in all four determinants being detected in the sialoprotein layer, while J was found in the lipoprotein as well. All antigens were relatively weak, being detected in inhibition strengths of 10.0 to 1.25 mg/ml.

摘要

从六头不同牛采集的红细胞产生的幽灵细胞用丁醇和吡啶进行降解。在这六头牛中共有38种不同的抗原决定簇可供研究，F、V、J和L是两种降解方法产生的亚组分中仅检测到的特异性抗原。丁醇降解后，V、J和L抗原存在于可溶性蛋白组分中，而F存在于不溶性蛋白中。吡啶消化导致在唾液蛋白层中检测到所有四种决定簇，同时在脂蛋白中也发现了J。所有抗原都相对较弱，在10.0至1.25mg/ml的抑制强度下被检测到。