Wigand R
Arch Virol. 1975;49(4):323-8. doi: 10.1007/BF01318241.
Two terminal dilution purifications in HEK cells were performed with adenovirus prototypes of types 1 to 7, 12, 16, 19 and 9-15, and one terminal dilution with a freshly isolated type 8 strain. The antisera prepared against viruses after terminal dilution neutralized the original and the purified virus to the same extent, indicating the antigenic purity of the respective prototypes. Cross-reactions in neutralization with other types within the subgroup were also identical. Only the prototype of type 16 (Ch 79) showed a cross-neutralization with type 4, while four type 16 wild strains showed cross-neutralization with type 14, but none with type 4. The prototype (CH 79) is a "prime strain" in relation to the type 16 wild strains.
在人胚肾（HEK）细胞中对1至7型、12型、16型、19型以及9至15型腺病毒原型进行了两次终点稀释纯化，并对新分离的8型毒株进行了一次终点稀释。终点稀释后针对病毒制备的抗血清对原始病毒和纯化病毒的中和程度相同，这表明各个原型的抗原纯度。在亚组内与其他型别之间的中和交叉反应也相同。只有16型原型株（Ch 79）与4型有交叉中和反应，而4株16型野生毒株与14型有交叉中和反应，但与4型无交叉中和反应。16型原型株（CH 79）相对于16型野生毒株而言是“原株”。
