Vero细胞培养物对人腺病毒的敏感性。

The susceptibility of Vero cell cultures for human adenoviruses.

作者信息

Hasler P, Wigand R

机构信息

Nationales Referenzzentrum für Adenoviren, Universität des Saarlandes, Homburg (Saar), Federal Republic of Germany.

出版信息

Med Microbiol Immunol. 1978;164(4):267-75. doi: 10.1007/BF02125495.

DOI:10.1007/BF02125495

PMID:45601

Abstract

Human adenoviruses 1 to 28 were shown to produce a cytopathic effect in Vero cell cultures. Viruses of subgroups III and IV (Ad 1, 2, 5, 6, 12, and 18) were readily passaged in Vero cell cultures and were produced in high amounts. This was also found for Ad 11, 16, and 21, while Ad 3, 4, and 7 showed a lower degree of multiplication and Ad 14 could not be passed serially. For Ad 8, 26, 27, 20, 25, and 28, a multiplication in Vero cells could not be proved, while the remaining serotypes of subgroup II showed a moderate degree of multiplication. The sensitivity of Vero cells to small amounts of virus was lower than that of HeLa cells. No adaption of adenoviruses to Vero cells after 5 Vero passages was observed. Attempts to enhance virus multiplication by coinfection with SV40 failed.

摘要

已证明人类腺病毒1至28型可在非洲绿猴肾细胞（Vero）培养物中产生细胞病变效应。III和IV亚组的病毒（腺病毒1、2、5、6、12和18型）很容易在Vero细胞培养物中传代，并大量产生。腺病毒11、16和21型也有此现象，而腺病毒3、4和7型的增殖程度较低，腺病毒14型无法连续传代。对于腺病毒8、26、27、20、25和28型，未证明其在Vero细胞中有增殖，而II亚组的其余血清型表现出中等程度的增殖。Vero细胞对少量病毒的敏感性低于HeLa细胞。在Vero细胞上传代5次后，未观察到腺病毒对Vero细胞的适应性。通过与猴空泡病毒40（SV40）共感染来增强病毒增殖的尝试失败了。