Lostia O, Costantini A, Jori D, Lorenzini P
Boll Ist Sieroter Milan. 1979 Mar 31;58(1):5-24.
The aim of the present paper is that of developing a technique which exploiting a commercial RIA Kit (Ausria II - Abbott) could be employed for the detection of HBsAg also in products different from serum or plasma, such as hemoderivates, even when as negative control is employed the kit, made up of a pool of HBsAg and HBsAb negative plasma. In order to lower the probability of false positive and false negatives the Authors employed, in parallel with the normal Abbott technique, a modified technique, which involves the presaturation of the polystyrene beads with human serum albumin solution. This technique showed to increase the sensitivity relevant to the kit itself. The technique with successive contacts employed by the Authors allowed the carrying out of the confirmation test for those blood derivatives also, which tend to clot, both due to the treatments and for the addition of the reagents employed in the confirmation test. In order the get a better garantee in classifying as "non-reactive" a sample the Authors expect it to be timely to carry out, in addition to the direct test, a confirmation test. Samples with titrated HBsAg additions and showing C/N > 1.5 in the direct test, turned out reactive to the confirmation test. Recovery tests show, even considering the variability of the sensitivity of the kit, according to the batch and the date of use, an indication of the sensitivity of the RIA method for HBsAg detection in hemoderivatives.
本文的目的是开发一种技术，该技术利用商业RIA试剂盒（Ausria II - Abbott），即使在以由HBsAg和HBsAb阴性血浆池组成的试剂盒作为阴性对照时，也可用于检测血清或血浆以外的产品（如血液衍生物）中的HBsAg。为了降低假阳性和假阴性的概率，作者在采用常规雅培技术的同时，还采用了一种改良技术，该技术涉及用人血清白蛋白溶液对聚苯乙烯珠进行预饱和。该技术显示出提高了与试剂盒本身相关的灵敏度。作者采用的连续接触技术也允许对那些因处理以及确认试验中所用试剂的添加而易于凝固的血液衍生物进行确认试验。为了在将样本分类为“无反应性”时获得更好的保证，作者期望除直接试验外，及时进行确认试验。在直接试验中添加了经滴定的HBsAg且C/N > 1.5的样本，在确认试验中显示为反应性。回收率试验表明，即使考虑到试剂盒灵敏度随批次和使用日期的变化，也能表明RIA法检测血液衍生物中HBsAg的灵敏度。
