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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1970 Jul;118(3):405-8. doi: 10.1042/bj1180405.

The reaction of d-2-hydroxy acid dehydrogenase with d-lactate and 2,6-dichlorophenol-indophenol (DCIP) at pH8.6 yields reciprocal plots of 1/rate versus 1/[d-lactate], at different DCIP concentrations, which appear to be parallel. However, at pH7.55, or in the presence of the competitive inhibitor oxalate at pH8.6, the plots are convergent. This is inconsistent with the mechanism previously proposed for this enzyme. 2. The pattern of inhibition by the product, pyruvate, is consistent with either an Ordered mechanism or an Iso Theorell-Chance mechanism. 3. The observation that the enzyme forms a complex with d-lactate favours the Ordered reaction. In this, first d-lactate and then DCIP bind to the enzyme to form a ternary complex, from which pyruvate and reduced DCIP dissociate in that order.

在pH8.6条件下，d - 2 - 羟基酸脱氢酶与d - 乳酸和2,6 - 二氯酚靛酚（DCIP）反应，在不同DCIP浓度下，得到1/速率对1/[d - 乳酸]的倒数图，这些图似乎是平行的。然而，在pH7.55时，或在pH8.6条件下存在竞争性抑制剂草酸盐时，这些图是收敛的。这与先前提出的该酶作用机制不一致。2. 产物丙酮酸的抑制模式与有序机制或Iso Theorell - Chance机制一致。3. 酶与d - 乳酸形成复合物这一观察结果支持有序反应。在此反应中，首先d - 乳酸然后DCIP与酶结合形成三元复合物，丙酮酸和还原型DCIP按此顺序从中解离。

Biochem J. 1970 Jul;118(3):405-8. doi: 10.1042/bj1180405.

The reaction of d-2-hydroxy acid dehydrogenase with d-lactate and 2,6-dichlorophenol-indophenol (DCIP) at pH8.6 yields reciprocal plots of 1/rate versus 1/[d-lactate], at different DCIP concentrations, which appear to be parallel. However, at pH7.55, or in the presence of the competitive inhibitor oxalate at pH8.6, the plots are convergent. This is inconsistent with the mechanism previously proposed for this enzyme. 2. The pattern of inhibition by the product, pyruvate, is consistent with either an Ordered mechanism or an Iso Theorell-Chance mechanism. 3. The observation that the enzyme forms a complex with d-lactate favours the Ordered reaction. In this, first d-lactate and then DCIP bind to the enzyme to form a ternary complex, from which pyruvate and reduced DCIP dissociate in that order.

在pH8.6条件下，d - 2 - 羟基酸脱氢酶与d - 乳酸和2,6 - 二氯酚靛酚（DCIP）反应，在不同DCIP浓度下，得到1/速率对1/[d - 乳酸]的倒数图，这些图似乎是平行的。然而，在pH7.55时，或在pH8.6条件下存在竞争性抑制剂草酸盐时，这些图是收敛的。这与先前提出的该酶作用机制不一致。2. 产物丙酮酸的抑制模式与有序机制或Iso Theorell - Chance机制一致。3. 酶与d - 乳酸形成复合物这一观察结果支持有序反应。在此反应中，首先d - 乳酸然后DCIP与酶结合形成三元复合物，丙酮酸和还原型DCIP按此顺序从中解离。