对纯化状态及完整红细胞中乳酸脱氢酶的研究。
Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.
作者信息
Simpson R J, Brindle K M, Brown F F, Campbell I D, Foxall D L
出版信息
Biochem J. 1982 Mar 15;202(3):581-7. doi: 10.1042/bj2020581.
Abstract
Lactate dehydrogenase in intact erythrocytes was studied by observing isotope exchange between lactate and pyruvate by p.m.r. The inhibition of the enzyme in intact cells by both oxalate and pyruvate was found to be similar to that of the purified enzyme. The activity of the enzyme in intact cells indicates that the free solution NAD+ + NADH concentration in erythrocytes is about 10 microM whereas the total extractable NAD+ + NADH is about 80 nmol/ml of cell water.
摘要
通过核磁共振观察乳酸和丙酮酸之间的同位素交换，对完整红细胞中的乳酸脱氢酶进行了研究。发现草酸盐和丙酮酸对完整细胞中该酶的抑制作用与纯化酶的抑制作用相似。完整细胞中该酶的活性表明，红细胞中游离溶液的NAD⁺ + NADH浓度约为10微摩尔，而可提取的总NAD⁺ + NADH约为每毫升细胞水80纳摩尔。
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