Belanger L, Hamel D, Dufour D, Pouliot M
Clin Chem. 1976 Feb;22(2):198-204.
We report the development of a double-antibody system for enzyme immunoassay of human alpha 1-fetoprotein. Equilibrium and sequential saturation procedures were evaluated and compared with a similar double-antibody radioimmunoassay. With our method, 3 mug of alpha 1-fetoprotein per liter can be detected. The dose-response curve covers a 100-fold range of analyte concentrations. Within-run and between-run coefficients of variation are respectively 6.9% and 10.0%. Analytical recovery of added antigen is 99.5 +/- 10.6%. Results are routinely obtained in 24 h but can be obtained in less than 8 h. Alpha 1-fetoprotein was measured in a variety of clinical samples, including sera from 488 pregnant women. The advantages of the present method over radioimmunoassay and other enzyme immunoassay procedures are discussed.
我们报告了一种用于人甲胎蛋白酶免疫测定的双抗体系统的开发。对平衡和顺序饱和程序进行了评估，并与类似的双抗体放射免疫测定进行了比较。用我们的方法，每升可检测到3微克甲胎蛋白。剂量反应曲线涵盖了100倍的分析物浓度范围。批内和批间变异系数分别为6.9%和10.0%。添加抗原的分析回收率为99.5±10.6%。常规情况下24小时可获得结果，但也可在不到8小时内获得。在包括488名孕妇血清在内的各种临床样本中检测了甲胎蛋白。讨论了本方法相对于放射免疫测定和其他酶免疫测定方法的优点。
