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[在不同载体上进行电泳后通过免疫化学方法鉴定蛋白质(作者译)]

[Identification of proteins by immunochemical methods after electrophoretic runs on different supports (author's transl)].

作者信息

Groulade J, Denis M C, Aveynier E

出版信息

Clin Chim Acta. 1976 Mar 1;67(2):199-206. doi: 10.1016/0009-8981(76)90260-6.

Abstract

The determination of the location of serum proteins after electrophoresis on various supports and after immunoelectrophoresis has been achieved by immunochemical methods with specific antisera. The present study describes the technique, gives details of various identified fractions and presents some figures. The method allows us to study the constitution of the "beta-gamma block" observed on paper and acetate supports after electrophoresis of cirrhotic sera and to superimpose the patterns of apparently distinct fractions on polyacrylamide gel.

摘要

通过使用特异性抗血清的免疫化学方法,已实现了对血清蛋白在各种支持物上进行电泳后以及免疫电泳后的定位测定。本研究描述了该技术,给出了各种已鉴定组分的详细信息,并呈现了一些数据。该方法使我们能够研究肝硬化血清电泳后在滤纸和醋酸纤维素支持物上观察到的“β-γ区带”的组成,并将明显不同组分的图谱叠加在聚丙烯酰胺凝胶上。

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