Link E, Schmidt-Wiederkehr P
Clin Chim Acta. 1976 Jun 15;69(3):447-55. doi: 10.1016/0009-8981(76)90118-2.
A modification of the Laurell method is described which makes it possible to measure very small amounts of proteins. The use of the intermediate gel technique allows the simultaneous determination of two or more proteins by one-dimension electrophoresis. The method is useful for differentiation of organ-specific proteins from serum proteins, for identification of identical and not-identical antibodies in antisera and for checking protein products and antisera for their purity.
本文描述了一种对劳雷尔方法的改进,该改进使得测量极少量蛋白质成为可能。中间凝胶技术的使用允许通过一维电泳同时测定两种或更多种蛋白质。该方法可用于区分血清蛋白中的器官特异性蛋白、鉴定抗血清中相同和不同的抗体以及检查蛋白质产物和抗血清的纯度。