Bernanke D H, Markwald R R
Tex Rep Biol Med. 1979;39:271-85.
To initiate the experimental exploration of the role of extracellular macromolecules in influencing developmental events in the heart, a 3-dimensional substrate culture model of the developing cardiac cushion was devised. One cardiac jelly component, hyaluronic acid, was tested for its effects on morphology and migratory capacity of cushion tissue cells within the collagen matrix substrate. Hyaluronate treatment resulted in: (1) an increase in the number and extent of filopodia, reflected as an increase in cell surface area, and (2) an increase in migratory capacity, reflected as an increase in maximum depth to which cells migrate through the collagen lattice. These results suggest one major role for hyaluronate in the early cardiac cushion is the promotion of a high level of motility capability in the newly seeded cushion tissue cells, required for the key event of cell migration across the developing cushion.
为了启动对细胞外大分子在影响心脏发育事件中作用的实验探索，设计了一种发育中心脏垫的三维基质培养模型。测试了一种心脏胶成分透明质酸对胶原基质底物中垫组织细胞形态和迁移能力的影响。透明质酸盐处理导致：（1）丝状伪足的数量和长度增加，表现为细胞表面积增加；（2）迁移能力增加，表现为细胞穿过胶原晶格迁移的最大深度增加。这些结果表明，透明质酸盐在早期心脏垫中的一个主要作用是促进新接种的垫组织细胞具有高水平的运动能力，这是细胞迁移穿过发育中的垫这一关键事件所必需的。
