Schulze W, Wallukat G, Altankow A
Zentralinstitut für Herz-Kreislauf-Forschung, Akademie der Wissenschaften, DDR, Berlin-Buch.
Acta Histochem Suppl. 1990;39:461-4.
Extracellular collagen from rat tail (types 1 and 3) is a suitable matrix for isolated neonatal heart cell cultivation. The fine structure and the physiological response to isoproterenol for instance is similar for neonatal cardiomyocytes cultured in the medium Sm 20-1 with or without collagen matrix. For electron microscopical routine preparation it is easier to use matrix-grown cardiomyocytes because additional preparation steps (centrifugation) are unnecessary.
来自大鼠尾巴的细胞外胶原蛋白（1型和3型）是用于分离的新生心脏细胞培养的合适基质。例如，在添加或不添加胶原蛋白基质的Sm 20-1培养基中培养的新生心肌细胞，其精细结构和对异丙肾上腺素的生理反应是相似的。对于电子显微镜常规制备，使用基质生长的心肌细胞更容易，因为不需要额外的制备步骤（离心）。
