Poulos A
J Lipid Res. 1970 Sep;11(5):496-8.
A method for the preparation of 1-alkyl 2-acyl glycero-3-phosphorylcholine, labeled in the 2-position with oleic acid-1-(14)C is described. This method, based on the acylation of a 1-alkyl glycero-3-phosphorylcholine-CdCl(2) complex which is, in turn, formed from 1-alk-1-enyl glycero-3-phosphorylcholine by hydrogenation and subsequent reaction with cadmium chloride, offers a relatively simple means of preparing a labeled saturated ether phospholipid suitable for use in metabolic studies.
描述了一种制备在2位用油酸-1-(14)C标记的1-烷基2-酰基甘油-3-磷酸胆碱的方法。该方法基于1-烷基甘油-3-磷酸胆碱-CdCl₂络合物的酰化反应,而该络合物又是由1-烯基甘油-3-磷酸胆碱通过氢化以及随后与氯化镉反应形成的,它提供了一种相对简单的制备适用于代谢研究的标记饱和醚磷脂的方法。