Morrell F, Tsuru N
Electroencephalogr Clin Neurophysiol. 1976 Jan;40(1):1-11. doi: 10.1016/0013-4694(76)90174-7.
The present report is the first demonstration of the kindling phenomenon in a non-mammalian, poikilothermic species. Brief (2 sec) localized electrical stimulation was delivered to the hippocampal cortex of one hemisphere of the partially paralyzed bullfrog, Rana catesbeiana, at current levels just sufficient to elicit brief after-discharge (AD) on first application. Succeeding stimulations, once per hour, gave rise to more and more prolonged AD involving first the side stimulated (1 degree focus) and secondly the opposite hemisphere (2 degree focus). In addition to these stimulus-dependent alterations, after several hours spontaneous, paroxysmal discharges occurred in interstimulus intervals. They appeared first in the stimulated hemisphere, then as evoked spikes in the secondary hemisphere, and finally independently on the two sides. The waveshape of the spontaneous epileptiform potentials exhibited an extraordinary constancy from complex to complex which was specific to each animal. Such wave-shape coherence implies a constancy to both the anatomical substrate and the sequency of synaptic engagement each time spontaneous paroxysmal activity invades the network. The model is especially suitable for analysis of temperature-dependent biochemical events responsible for this functional transformation.
本报告首次证明了在非哺乳动物、变温动物物种中存在点燃现象。对部分瘫痪的牛蛙（牛蛙属）一侧半球的海马皮层进行短暂（2秒）局部电刺激，电流强度刚好足以在首次施加时引发短暂的后放电（AD）。随后每小时进行一次刺激，引发的AD越来越长，首先涉及受刺激的一侧（一级焦点），其次是对侧半球（二级焦点）。除了这些依赖于刺激的变化外，在刺激间隔期间还会出现数小时的自发阵发性放电。它们首先出现在受刺激的半球，然后在次级半球表现为诱发尖峰，最后在两侧独立出现。自发癫痫样电位的波形在每次发作时都表现出非同寻常的稳定性，这对每只动物来说都是特定的。这种波形一致性意味着每次自发阵发性活动侵入网络时，解剖学基质和突触参与顺序都具有稳定性。该模型特别适合分析导致这种功能转变的温度依赖性生化事件。
