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[膜蛋白在Triton-X 100中的色谱分析。I)膜蛋白在Triton-X 100中的溶解]

[Chromatography of membrane proteins in Triton-X 100. I) Solubilization of membrane proteins in Triton-X 100].

作者信息

Landi F, Landi V, Cardillo A, Fabrizi G

出版信息

Boll Soc Ital Biol Sper. 1979 Aug 30;55(16):1637-43.

PMID:553548
Abstract

In their study of the chemical and physical characteristics of membrane protein, the Authors have directed their attention to Triton-X 100. The hypothesis is that this detergent would have two antagonistic effects: on the one hand it would delimit the membrane fostering the formation of unstable complexes, on the other it would establish unstable intermediate complexes fostering the formation of soluble proteic complexes.

摘要

在对膜蛋白的化学和物理特性进行研究时,作者们将注意力集中在了曲拉通-X 100上。提出的假设是,这种去污剂会产生两种相反的作用:一方面,它会界定膜,促进不稳定复合物的形成;另一方面,它会形成不稳定的中间复合物,促进可溶性蛋白质复合物的形成。

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