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[膜蛋白在Triton-X 100中的色谱分析。II)膜蛋白在与琼脂糖偶联的伴刀豆球蛋白A上于Triton-X 100中的亲和色谱分析]

[Chromatography of membrane proteins in Triton-X 100. II) Affinity chromatography of membrane proteins on Con-A linked to Sepharose in Triton-X 100].

作者信息

Landi F, Landi V, Cardillo A, Fabrizi G

出版信息

Boll Soc Ital Biol Sper. 1979 Aug 30;55(16):1644-50.

PMID:553549
Abstract

The Authors have made chromatographs using Con-A Sefaroso columns, varying the percent of Triton-X 100 and the proteic concentration. With proteic concentrations varying from 0.4 to 4mg/ml and in solutions containing 0.5 to 1% of Triton-X 100 with the pH value remaining constant at 8 - solubilization of membrane protein does occur.

摘要

作者使用伴刀豆球蛋白A琼脂糖柱制作了色谱图,改变了吐温-X 100的百分比和蛋白质浓度。蛋白质浓度在0.4至4mg/ml之间变化,且在含有0.5%至1%吐温-X 100的溶液中,pH值保持在8不变时,膜蛋白确实会发生溶解。

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