Panza N, Mondola P, Falconi C, Cifaldi S, Santangelo F
Boll Soc Ital Biol Sper. 1979 Dec 30;55(24):2635-41.
The estrogen receptors presence or absence in the thymus gland represents the basic element to assert or to exclude any estrogenic action in the thymus. Now we have measured the presence of cytoplasmatic estrogen receptors in the female rabbits thymus, using some methods (sucrose gradient, protamine sulphate, dextran-coated charcoal). Finally, we have investigated, using the sucrose gradient assay, the nuclear ER presence in 1000g thymic pellet. The results exclude high levels of estrogen-binding proteins while don't suggest their total absence in the female rabbit thymus.
胸腺中雌激素受体的存在与否是确定或排除胸腺中任何雌激素作用的基本要素。现在我们使用了一些方法(蔗糖梯度法、硫酸鱼精蛋白法、葡聚糖包被活性炭法)来测定雌性兔胸腺中细胞质雌激素受体的存在情况。最后,我们使用蔗糖梯度分析法研究了1000g胸腺沉淀中细胞核雌激素受体的存在情况。结果排除了高水平雌激素结合蛋白的存在,但并未表明其在雌性兔胸腺中完全不存在。