Haruki Y, Seiki K, Enomoto T, Fujii H, Sakabe K
Tokai J Exp Clin Med. 1983 Jan;8(1):31-9.
Estrogen binding components have been characterized in cytosols from lymphocyte fractions and non-lymphocyte fractions of the thymus and mesenteric lymph node of ovariectomized rats. The cytosol from the non-lymphocyte fraction of the thymus contained high affinity-specific binding estrogen receptor (equilibrium dissociation constant .=. 3 X 10(-10) M, number of binding sites .=. 10 fmol/mg protein) with a sedimentation coefficient of 8 S. The receptor showed specific binding for estradiol-17 beta, estrone and estriol, but not for corticosterone, progesterone or testosterone. Only lower estrogen binding was observed in the cytosols from the lymphocyte fraction of both tissues and the non-lymphocyte fraction of the lymph node. Morphological changes of the thymus in ovariectomized rats after administration of estradiol benzoate were also observed. Clustered vacuoles with incomplete microvilli in the epithelial cells increased in number and in size after estrogen treatment. These findings suggest that the effect of estrogen on the thymus is mediated via the cytoplasmic estrogen receptor in the non-lymphocytes, probably thymic epithelial cells.
在去卵巢大鼠的胸腺和肠系膜淋巴结的淋巴细胞组分和非淋巴细胞组分的胞质溶胶中，已对雌激素结合成分进行了表征。胸腺非淋巴细胞组分的胞质溶胶含有高亲和力特异性结合雌激素受体（平衡解离常数=3×10⁻¹⁰M，结合位点数=10 fmol/mg蛋白质），沉降系数为8S。该受体对雌二醇-17β、雌酮和雌三醇表现出特异性结合，但对皮质酮、孕酮或睾酮无特异性结合。在两个组织的淋巴细胞组分以及淋巴结的非淋巴细胞组分的胞质溶胶中，仅观察到较低的雌激素结合。还观察了苯甲酸雌二醇给药后去卵巢大鼠胸腺的形态学变化。雌激素处理后，上皮细胞中具有不完全微绒毛的聚集空泡数量和大小增加。这些发现表明，雌激素对胸腺的作用是通过非淋巴细胞（可能是胸腺上皮细胞）中的细胞质雌激素受体介导的。
