假异氰染料染色的人类染色体的细胞化学研究
Cytochemical study of pseudoisocyanine stained human chromosomes.
作者信息
Vagner-Capodano A M, Pinna-Delgrossi M H, Stahl A
出版信息
Hum Genet. 1976 Jan 28;31(1):9-19. doi: 10.1007/BF00270394.
Human meiotic and mitotic chromosomes were studied with N-N' diethyl pseudoisocyanine stain. Following methylation and oxydation, the staining allowed microscopic observation of slides with both monochromatic light and fluorescence. In addition, stained preparations can be permanently conserved. Preceeded by diverse methods of chromosome denaturation or 5-BUDR incorporation, PIC lends itself to a large number of banding techniques. Cytochemical study of stained chromosomes demonstrated a certain PIC affinity for DNA although tests performed do not exclude the possibility of PIC reaction with certain proteins.
用N - N' - 二乙基假异氰染料对人类减数分裂和有丝分裂染色体进行了研究。经过甲基化和氧化后，这种染色使得在单色光和荧光下都能对载玻片进行显微镜观察。此外，染色后的标本可以永久保存。在采用多种染色体变性方法或掺入5 - 溴脱氧尿苷（5 - BUDR）之前，PIC适用于大量的显带技术。对染色染色体的细胞化学研究表明，PIC对DNA有一定的亲和力，尽管所进行的测试不排除PIC与某些蛋白质发生反应的可能性。
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