Zoncheddu A, Accomando R, Pertica M, Orunesu M
Ital J Biochem. 1979 Sep-Oct;28(5):335-44.
Phosphocellulose chromatography has been employed to characterize RNA polymerase I present in two different functional states in rat liver cells. The actively transcribing enzyme solubilized from nuclei appears to belong both to the IA and IB classes, whereas the non-transcribing enzyme present in the cytoplasmic fraction has been found to belong only to the IA class. Indirect and direct evidence indicates, however, that in isolated nuclei only the IB form is to be regarded as the physiological form of the enzyme, the IA form arising as a procedural artefact during the extraction process. It may, therefore, be concluded that rat liver IA and IB RNA polymerase are to be strictly regarded as the non-transcribing and transcribing form of the enzyme, respectively.
磷酸纤维素色谱法已被用于表征大鼠肝细胞中处于两种不同功能状态的RNA聚合酶I。从细胞核中溶解出来的活跃转录酶似乎同时属于IA类和IB类，而细胞质部分中存在的非转录酶仅属于IA类。然而，间接和直接证据表明，在分离的细胞核中，只有IB形式被视为该酶的生理形式，IA形式是在提取过程中作为操作假象产生的。因此，可以得出结论，大鼠肝脏IA和IB RNA聚合酶应分别被严格视为该酶的非转录形式和转录形式。
