大鼠肝脏中单胺氧化酶的电子显微镜定位
Electron microscopic localization of monoamine oxidase in the rat liver.
作者信息
Yoo B Y, Oreland L
出版信息
Histochemistry. 1976 Feb 16;46(2):131-7. doi: 10.1007/BF02462737.
PMID:55404
Abstract
Two methods have been employed to localize monoamine oxidase activity in the cells of rat liver, using either 2-(2'-benzothiazolyl)-5-stryl-3-(4'-phtalhydrazidyl) tetrazolium chloride (BSPT) or ferricyanide as electron acceptor. With both methods monoamine oxidase activity was found both in the inner and the outer mitochondral membrane, although the outer membrane appeared the most probable location. In addition the BSPT method but not the ferricyanide method, revealed monoamine oxidase activity in the endoplasmatic reticulum. The results obtained by the two methods have been compared and are discussed in view of available biochemical data on monoamine oxidase.
摘要
已采用两种方法来定位大鼠肝脏细胞中的单胺氧化酶活性，一种方法是使用2 -（2'-苯并噻唑基）-5 - 苯乙烯基-3 -（4'-邻苯二甲酰肼基）四唑氯化物（BSPT），另一种方法是使用铁氰化物作为电子受体。两种方法均发现线粒体内膜和外膜都存在单胺氧化酶活性，不过外膜似乎是最可能的位置。此外，BSPT法而非铁氰化物法显示内质网中存在单胺氧化酶活性。已对两种方法获得的结果进行了比较，并根据有关单胺氧化酶的现有生化数据进行了讨论。
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Distribution of enzymes between subcellular fractions in animal tissues.动物组织中亚细胞组分间酶的分布
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Monoamine oxidase activity in membrane structures of rat liver cell.大鼠肝细胞膜结构中的单胺氧化酶活性
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Cytochemical differentiation between monoamine oxidase and other neuronal oxidases.单胺氧化酶与其他神经元氧化酶之间的细胞化学分化
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A method for histochemical demonstration of -glycerophosphateferricyanide oxidoreductase activity.一种用于组织化学显示α-甘油磷酸铁氰化物氧化还原酶活性的方法。
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Biochem Biophys Res Commun. 1968 Jun 28;31(6):901-7. doi: 10.1016/0006-291x(68)90537-8.
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The site of ferricyanide reduction by reductases within mitochondria as studied by electron microscopy.通过电子显微镜研究线粒体中还原酶还原铁氰化物的位点。
J Histochem Cytochem. 1968 Jan;16(1):49-57. doi: 10.1177/16.1.49.
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The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria.单胺氧化酶的亚线粒体定位。大鼠肝脏线粒体外膜的一种酶标记物。
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