分枝杆菌纯化原生质肽：一种结核菌素活性糖肽的化学成分
Purified protoplasmic peptides of mycobacteria: chemical composition of a tuberculin-active glycopeptide.
作者信息
Stottmeier K D, Beam R E, David H L, Shapira R, Farshy D C
出版信息
J Bacteriol. 1971 Jan;105(1):172-5. doi: 10.1128/jb.105.1.172-175.1971.
Abstract
A tuberculin-active glycopeptide containing eight different amino acids and glucose was isolated from the protoplasm of Mycobacterium tuberculosis. A molecular weight of 4,000 to 5,000 was established by Sephadex gel filtration; other analyses showed a peptide to carbohydrate ratio of 9:1. These observations suggest a tentative composition of 3 to 4 residues of glucose, 12 residues each of aspartic and glutamic acids, 3 residues each of lysine, glycine, and serine, and 1 residue each of arginine, threonine, and alanine.
摘要
从结核分枝杆菌的原生质中分离出一种含有8种不同氨基酸和葡萄糖的具有结核菌素活性的糖肽。通过葡聚糖凝胶过滤确定其分子量为4000至5000；其他分析表明肽与碳水化合物的比例为9:1。这些观察结果提示其初步组成为3至4个葡萄糖残基、天冬氨酸和谷氨酸各12个残基、赖氨酸、甘氨酸和丝氨酸各3个残基以及精氨酸、苏氨酸和丙氨酸各1个残基。
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本文引用的文献
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The immunology and chemistry of tuberculin. I. The isolation of dialyzable and nondialyzable tuberculin-active components from unheated BCG culture filtrates.结核菌素的免疫学与化学。I. 从未加热的卡介苗培养滤液中分离可透析和不可透析的结核菌素活性成分。
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Purified protoplasmic peptides of mycobacteria: isolation of species-specific peptides from protoplasm of mycobacteria.分枝杆菌的纯化原生质肽：从分枝杆菌原生质中分离种特异性肽。
J Bacteriol. 1969 Oct;100(1):195-200. doi: 10.1128/jb.100.1.195-200.1969.
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Purified protoplasmic peptides of mycobacteria: in vivo and in vitro comparison of the species specificity of purified protoplasmic peptides and purified protein derivatives of mycobacterial culture filtrates.分枝杆菌的纯化原生质肽：纯化原生质肽与分枝杆菌培养滤液纯化蛋白衍生物的种属特异性的体内和体外比较
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