Beam R E, Stottmeier K D, Kubica G P
J Bacteriol. 1969 Oct;100(1):195-200. doi: 10.1128/jb.100.1.195-200.1969.
Infections with mycobacteria other than tubercle bacilli are responsible for a variable percentage of cross-reactions to tuberculin. Two major suggestions for circumventing this problem have been made: the first, development of a quantitative tuberculin test, is based on the fact that most cross-reactions are smaller than those caused by true tuberculous infections; the second, preparation of purified skin test antigens from other mycobacteria, is based on the hope that greater specificity will be displayed by homologous sensitin. Effort so far has been focused on the culture filtrates as the source of antigen. This article describes the preparation of low molecular weight purified protoplasmic peptides (PPP) of specificity and sensitivity superior to purified protein derivatives.
除结核杆菌外的分枝杆菌感染导致了对结核菌素不同比例的交叉反应。针对解决这一问题主要提出了两点建议：第一，开发一种定量结核菌素试验，其依据是大多数交叉反应比真正的结核感染所引起的反应要小；第二，从其他分枝杆菌制备纯化的皮肤试验抗原，其基于的希望是同源敏感素能表现出更高的特异性。迄今为止，工作一直集中在将培养滤液作为抗原来源。本文描述了特异性和敏感性优于纯化蛋白衍生物的低分子量纯化原生质肽（PPP）的制备方法。
