Benzo C A, Nemeth A M
J Cell Biol. 1971 Feb;48(2):235-47. doi: 10.1083/jcb.48.2.235.
The 5 day chick embryo liver cell still lacks many of the ultrastructural features of the adult liver cell. During organ culture on rafts over Eagle's medium, it develops electron-opaque mitochondria with granules, biliary microvilli, and compact Golgi complexes containing what appears to be secretory material. Rough ER proliferates and free ribosomes become bound to membrane. Thus, the 5 day cell, exposed only to simple nutrients (glucose, amino acids, vitamins) develops the general appearance of the adult liver cell except for the continued absence of smooth ER and glycogen. The significance of this incomplete differentiation and the factors controlling development are discussed in the light of accompanying metabolic changes.
5日龄鸡胚肝细胞仍缺乏成年肝细胞的许多超微结构特征。在伊格尔培养基上的筏式器官培养过程中，它会发育出带有颗粒的电子不透明线粒体、胆小管微绒毛以及含有似乎是分泌物质的致密高尔基体复合物。粗面内质网增殖，游离核糖体与膜结合。因此，仅暴露于简单营养物质（葡萄糖、氨基酸、维生素）的5日龄细胞，除了持续缺乏滑面内质网和糖原外，呈现出成年肝细胞的一般外观。根据伴随的代谢变化，讨论了这种不完全分化的意义以及控制发育的因素。
