转化能力对流感嗜血杆菌中双重感染双溶原菌形成的影响。
Influence of transformability on the formation of superinfection double lysogens in Haemophilus influenzae.
作者信息
Stuy J H, Hoffmann J F
出版信息
J Virol. 1971 Jan;7(1):127-36. doi: 10.1128/JVI.7.1.127-136.1971.
Abstract
Superinfection of growing (nontransformable) cells of defectively lysogenic strains of Haemophilus influenzae with wild-type or with mutant phage HP1 resulted in a number of double lysogens and a small number of monolysogens with altered prophage. The double lysogens were identified by analysis of their monolysogenic segregants and by examining their deoxyribonucleic acid in certain test crosses. The results indicate that the majority had been formed by insertion of the infecting phage genome within the resident prophage. Superinfection of transformable bacteria gave rise to cells with altered prophages (presumably transformants) and to double lysogens which had gained or lost wild-type prophage loci.
摘要
用野生型或突变噬菌体HP1对流感嗜血杆菌缺陷溶原性菌株的生长（不可转化）细胞进行超感染，产生了许多双溶原菌和少量前噬菌体发生改变的单溶原菌。通过分析其单溶原性分离株并在某些测试杂交中检查其脱氧核糖核酸来鉴定双溶原菌。结果表明，大多数双溶原菌是由感染噬菌体基因组插入驻留前噬菌体中形成的。可转化细菌的超感染产生了前噬菌体发生改变的细胞（可能是转化体）以及获得或失去野生型前噬菌体位点的双溶原菌。
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