甘油对流感嗜血杆菌转染的影响。
Effect of glycerol on Haemophilus influenzae transfection.
作者信息
Stuy J H
出版信息
J Bacteriol. 1986 Apr;166(1):285-9. doi: 10.1128/jb.166.1.285-289.1986.
Abstract
Competent Haemophilus influenzae bacteria were exposed to purified phage HP1 DNA and then plated for transfectants (PFU). When 32% (final concentration) glycerol was added before plating, between 10- and 100-fold more transfectants were observed. Glycerol had no significant effect on transfection with DNA from single or tandem double lysogens. It also had little effect on transformation with chromosomal DNA or on transformation of defective HP1 lysogens with phage HP1 DNA. It was concluded that glycerol induced the release of adsorbed linear double-stranded DNA into the interior of the cells.
摘要
将有活性的流感嗜血杆菌暴露于纯化的噬菌体HP1 DNA中，然后接种以筛选转染体（噬菌斑形成单位）。在接种前加入32%（终浓度）的甘油后，观察到转染体数量增加了10至100倍。甘油对来自单溶源菌或串联双溶源菌的DNA转染没有显著影响。它对染色体DNA转化或用噬菌体HP1 DNA对缺陷型HP1溶源菌的转化也几乎没有影响。得出的结论是，甘油诱导吸附的线性双链DNA释放到细胞内部。
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