戊二醛对人红细胞固定作用的定量分析。
Quantitation of human red blood cell fixation by glutaraldehyde.
作者信息
Morel F M, Baker R F, Wayland H
出版信息
J Cell Biol. 1971 Jan;48(1):91-100. doi: 10.1083/jcb.48.1.91.
Abstract
The uptake of glutaraldehyde by human red blood cells has been measured as a function of time by a freezing point osmometer. The rate of attachment of glutaraldehyde to the cell proteins is high over the first hour, declining to zero over a period of a few days. The number of glutaraldehyde molecules cross-linking with each hemoglobin molecule is of the order of 200, in reasonable agreement with the calculated number of attachment sites. The cell membrane is immediately highly permeable to glutaraldehyde. Selective permeability to ions is lost during fixation. Ionic equilibrium is obtained only after a few hours. An optimum fixation technique for shape preservation is suggested.
摘要
已通过冰点渗透压计测量了人红细胞对戊二醛的摄取量随时间的变化。在最初的一小时内，戊二醛与细胞蛋白质的附着速率很高，在几天的时间内降至零。与每个血红蛋白分子交联的戊二醛分子数量约为200，与计算出的附着位点数量合理相符。细胞膜对戊二醛立即具有高渗透性。在固定过程中离子选择性渗透性丧失。仅在数小时后才达到离子平衡。提出了一种用于保持形状的最佳固定技术。
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