成年和新生大鼠及豚鼠肾皮质和髓质的耗氧率及无氧糖酵解率

Rates of oxygen consumption and of anaerobic glycolysis in renal cortex and medulla of adult and new-born rats and guinea-pigs.

Dicker S E, Shirley D G

J Physiol. 1971 Jan;212(1):235-43. doi: 10.1113/jphysiol.1971.sp009319.

Abstract

Rates of oxygen uptake and of anaerobic glycolysis were estimated in slices from the renal cortex and medulla (a) of adult rats and guinea-pigs, (b) of new-born (1-, 5- and 21-day-old) rats and of guinea-pigs of 1, 12, 21, 24 and 120 hr age.2. In the adult rat, Q(O2) values for the cortex were 12.55 +/- 0.20 (22) and for the medulla: 8.56 +/- 0.17 (22) mul./hr.mg dry weight, while in the new-born rat (24 hr old) they were 10.99 +/- 0.46 (12) and 9.33 +/- 0.18 (9) mul./hr.mg dry weight respectively.3. Values for Q(CO2) (N2) (anaerobic glycolysis) in the 14 hr old new-born rat were in the renal cortex 9.65 +/- 0.35 (5) and in the medulla 7.39 +/- 0.43 (5) mul./hr.mg dry weight; while in the adult they were 2.25 +/- 0.08 (16) and 5.76 +/- 0.14 (16) mul./hr.mg dry weight, respectively.4. In the adult guinea-pig values for Q(CO2) (N2) were of the same order as in the adult rat, though the rate of O(2) uptake was for the cortex 8.12 +/- 0.22 (12) and for the medulla 5.02 +/- 0.23 (11) mul./hr.mg dry weight.5. Though the Q(O2) values in the renal cortex and medulla were smaller in the 1 hr old new-born guinea-pig, they were already increasing in the 12 hr old neonate.6. The results are discussed in the light of enzyme changes occurring during the process of maturation of the nephron as indicated by histochemical observations.

摘要

对成年大鼠和豚鼠肾皮质与髓质切片，以及新生（1日龄、5日龄和21日龄）大鼠和1小时、12小时、21小时、24小时及120小时龄豚鼠的肾皮质与髓质切片的氧摄取率和无氧糖酵解率进行了估算。
在成年大鼠中，皮质的Q(O2)值为12.55±0.20（22），髓质为8.56±0.17（22）微升/小时·毫克干重；而在新生大鼠（24小时龄）中，它们分别为10.99±0.46（12）和9.33±0.18（9）微升/小时·毫克干重。
14小时龄新生大鼠肾皮质的Q(CO2)(N2)（无氧糖酵解）值为9.65±0.35（5），髓质为7.39±0.43（5）微升/小时·毫克干重；而成年大鼠分别为2.25±0.08（16）和5.76±0.14（16）微升/小时·毫克干重。
在成年豚鼠中，Q(CO2)(N2)值与成年大鼠处于同一水平，尽管皮质的氧摄取率为8.12±0.22（12），髓质为5.02±0.23（11）微升/小时·毫克干重。
虽然1小时龄新生豚鼠肾皮质和髓质的Q(O2)值较小，但在12小时龄的新生儿中它们已经开始增加。
根据组织化学观察所示，结合肾单位成熟过程中发生的酶变化对结果进行了讨论。