Mollenhauer H H, Totten C
J Cell Biol. 1971 Mar;48(3):533-41. doi: 10.1083/jcb.48.3.533.
Two structurally distinct lipid vesicles are present in pea and bean cotyledons during the first few days of germination. Both were isolated by sucrose density gradient centrifugation without significant morphological changes. Lipid vesicles of one type were elongated into a sausage-like or flattened-saccular shape, and were interassociated into sheets which were usually one vesicle thick. These sheets remained intact during homogenization and centrifugation, because some of the lipid vesicles in the sheet were interconnected through their bounding membranes, and because there seemed to be a bonding substance between adjacent vesicles. These vesicles were called "composite" lipid vesicles to distinguish them from the more usual, or "simple," lipid vesicles of other plant and animal tissues. Lipid vesicles of the other type were usually larger than the composite lipid vesicles and were always spherical in form. These vesicles remained single and did not interassociate into sheets. They were probably equivalent to the simple lipid vesicles of other tissues.
在豌豆和菜豆的子叶萌发的最初几天，存在两种结构不同的脂质体。通过蔗糖密度梯度离心法将二者分离，且未发生明显的形态变化。一种类型的脂质体被拉长呈香肠状或扁平囊状，并相互连接形成通常为单层脂质体厚度的片层结构。这些片层在匀浆和离心过程中保持完整，这是因为片层中的一些脂质体通过其边界膜相互连接，而且相邻脂质体之间似乎存在一种结合物质。这些脂质体被称为“复合”脂质体，以区别于其他动植物组织中更常见的“简单”脂质体。另一种类型的脂质体通常比复合脂质体大，且始终呈球形。这些脂质体保持单个状态，不会相互连接形成片层。它们可能等同于其他组织中的简单脂质体。
