Division of Horticultural Research, CSIRO, G.P.O. Box 350, 5001, Adelaide, S.A., Australia.
Planta. 1973 Mar;109(1):61-72. doi: 10.1007/BF00385453.
Freeze-etching and thin-sectioning techniques have been used to study the structure of storage parenchyma cells of pea cotyledons in mature, air-dry seeds and in seeds after various periods of imbibition. Protein bodies in air-dry tissue were coated with a complex of convoluted structure, apparently rich in lipid. After brief periods of hydration this complex appeared to have expanded and to consist of a system of tubules, and after 24 h imbibition it was no longer associated with the protein bodies. Sheets of lipid bodies were associated with amyloplast surfaces and the plasmalemma. In non-imbibed tissue, the cell walls were wavy, but in imbibed tissue they were smooth. The amyloplast-envelope membranes characteristically had areas with parallel folds, and membrane particles of protein-body envelopes were occasionally aligned in orderly rows.
冷冻蚀刻和超薄切片技术已被用于研究成熟、风干的豌豆子叶贮藏组织细胞的结构，以及在不同吸胀阶段后的种子结构。在风干组织中，蛋白质体被一层复杂的卷曲结构包裹，显然富含脂质。在短暂的水合作用后，这种复合物似乎已经膨胀，并由一个管状系统组成，在 24 小时吸胀后，它不再与蛋白质体相关联。脂质体薄片与淀粉体表面和质膜相关联。在未吸胀的组织中，细胞壁是波浪状的，但在吸胀的组织中，细胞壁是光滑的。淀粉体-被膜的特征是具有平行折叠的区域，并且蛋白质体被膜的膜颗粒偶尔会排列成有序的行。
