棕色固氮菌中磷酸烯醇丙酮酸分支点的调控：丙酮酸激酶
Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: pyruvate kinase.
作者信息
Liao C L, Atkinson D E
出版信息
J Bacteriol. 1971 Apr;106(1):37-44. doi: 10.1128/jb.106.1.37-44.1971.
Abstract
Pyruvate kinase (EC 2.7.1.40) from Azotobacter vinelandii responds sharply to the adenylate energy charge, with a decrease in activity at high values of charge, as expected for an enzyme of an adenosine triphosphate-regenerating sequence. Glycolytic intermediates, especially glucose 6-phosphate, fructose 6-phosphate, and fructose-1,6-diphosphate, strongly stimulate the reaction and overcome the inhibition caused by high values of energy charge. Thus, the properties of this enzyme depend on interaction between energy charge and the concentrations of hexose phosphates. The properties of pyruvate kinase, together with those of phosphoenolpyruvate carboxylase, aspartokinase, and citrate synthase, seem adapted to provide appropriate partitioning of phosphoenolpyruvate between competing pathways in response to metabolic need.
摘要
来自棕色固氮菌的丙酮酸激酶（EC 2.7.1.40）对腺苷酸能荷有敏锐的反应，在高能荷值时活性降低，这对于三磷酸腺苷再生序列中的一种酶来说是预期的。糖酵解中间产物，尤其是6-磷酸葡萄糖、6-磷酸果糖和1,6-二磷酸果糖，能强烈刺激该反应并克服高能荷值引起的抑制作用。因此，这种酶的特性取决于能荷与己糖磷酸浓度之间的相互作用。丙酮酸激酶的特性，连同磷酸烯醇式丙酮酸羧化酶、天冬氨酸激酶和柠檬酸合酶的特性，似乎适合于根据代谢需求在竞争途径之间对磷酸烯醇式丙酮酸进行适当的分配。
相似文献
1
Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: pyruvate kinase.棕色固氮菌中磷酸烯醇丙酮酸分支点的调控：丙酮酸激酶
J Bacteriol. 1971 Apr;106(1):37-44. doi: 10.1128/jb.106.1.37-44.1971.
2
Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: phosphoenolpyruvate carboxylase.棕色固氮菌中磷酸烯醇丙酮酸分支点的调控：磷酸烯醇丙酮酸羧化酶
J Bacteriol. 1971 Apr;106(1):31-6. doi: 10.1128/jb.106.1.31-36.1971.
3
Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. II. Regulation of phosphoenolpyruvate carboxylase and pyruvate kinase.黄色短杆菌中三羧酸循环和乙醛酸循环的调节。II. 磷酸烯醇式丙酮酸羧化酶和丙酮酸激酶的调节
J Biochem. 1969 Sep;66(3):297-311. doi: 10.1093/oxfordjournals.jbchem.a129148.
4
Regulation of liver pyruvate kinase and the phosphoenolpyruvate crossroads.肝脏丙酮酸激酶与磷酸烯醇丙酮酸交叉点的调节
Eur J Biochem. 1970 Mar 1;13(1):45-54. doi: 10.1111/j.1432-1033.1970.tb00897.x.
5
Isolation and characterization of pyruvate carboxylase from Azotobacter vinelandii OP.从棕色固氮菌OP中分离和鉴定丙酮酸羧化酶。
Arch Biochem Biophys. 1974 Oct;164(2):641-54. doi: 10.1016/0003-9861(74)90076-9.
6
Leishmania donovani and L. braziliensis: function of pyruvate kinase.杜氏利什曼原虫和巴西利什曼原虫：丙酮酸激酶的功能
Exp Parasitol. 1977 Dec;43(2):407-13. doi: 10.1016/0014-4894(77)90047-9.
7
[Regulation of PEP-carboxylase of the facultative methylotrop Acetobacter sp. MB 58].[兼性甲基营养型醋酸杆菌MB 58中磷酸烯醇式丙酮酸羧化酶的调控]
Z Allg Mikrobiol. 1982;22(7):453-63. doi: 10.1002/jobm.3630220705.
8
Novel enzymic machinery for the metabolism of oxalacetate, phosphoenolpyruvate, and pyruvate in Pseudomonas citronellolis.香茅假单胞菌中草酰乙酸、磷酸烯醇丙酮酸和丙酮酸代谢的新型酶机制。
J Biol Chem. 1977 Feb 25;252(4):1257-63.
9
[Regulation of glycolytic transformation by the synthesis and degradation of enzymes].[酶的合成与降解对糖酵解转化的调节作用]
Z Naturforsch B. 1969 Dec;24(12):1544-9.
10
Regulation at the phosphoenolpyruvate branchpoint by the adenylate energy charge in Mytilus edulis.紫贻贝中腺苷酸能荷对磷酸烯醇丙酮酸分支点的调节作用。
Biochem Soc Trans. 1976;4(3):444-7. doi: 10.1042/bst0040444.
引用本文的文献
1
The K-Dependent and -Independent Pyruvate Kinases Acquire the Active Conformation by Different Mechanisms.依赖 K 和不依赖 K 的丙酮酸激酶通过不同的机制获得活性构象。
Int J Mol Sci. 2022 Jan 25;23(3):1347. doi: 10.3390/ijms23031347.
2
The contribution of two isozymes to the pyruvate kinase activity of Vibrio cholerae: One K+-dependent constitutively active and another K+-independent with essential allosteric activation.两种同工酶对霍乱弧菌丙酮酸激酶活性的贡献：一种依赖钾离子且组成型激活，另一种不依赖钾离子但需必需的别构激活。
PLoS One. 2017 Jul 7;12(7):e0178673. doi: 10.1371/journal.pone.0178673. eCollection 2017.
3
New insights on the mechanism of the K(+-) independent activity of crenarchaeota pyruvate kinases.奇古菌丙酮酸激酶K(+)非依赖性活性机制的新见解。
PLoS One. 2015 Mar 26;10(3):e0119233. doi: 10.1371/journal.pone.0119233. eCollection 2015.
4
Proteins from extremophiles as stable tools for advanced biotechnological applications of high social interest.来自极端微生物的蛋白质作为具有高度社会意义的先进生物技术应用的稳定工具。
J R Soc Interface. 2007 Apr 22;4(13):183-91. doi: 10.1098/rsif.2006.0174.
5
Pyruvate kinase, a possible regulatory enzyme in higher plants.丙酮酸激酶，高等植物中一种可能的调节酶。
Plant Physiol. 1973 Oct;52(4):312-7. doi: 10.1104/pp.52.4.312.
6
Use of physiological constraints to identify quantitative design principles for gene expression in yeast adaptation to heat shock.利用生理限制来确定酵母适应热休克过程中基因表达的定量设计原则。
BMC Bioinformatics. 2006 Apr 3;7:184. doi: 10.1186/1471-2105-7-184.
7
Physiological characterization of viable-but-nonculturable Campylobacter jejuni cells.空肠弯曲杆菌活的非可培养细胞的生理学特性
Appl Environ Microbiol. 1999 Mar;65(3):1110-6. doi: 10.1128/AEM.65.3.1110-1116.1999.
8
Glucose 6-phosphate activation of pyruvate kinase from Mycobacterium smegmatis.耻垢分枝杆菌丙酮酸激酶的6-磷酸葡萄糖激活作用
Biochem J. 1981 Feb 1;193(2):435-40. doi: 10.1042/bj1930435.
9
Purification and properties of pyruvate kinase from Streptococcus mutans.变形链球菌丙酮酸激酶的纯化及性质
J Bacteriol. 1982 Jan;149(1):299-305. doi: 10.1128/jb.149.1.299-305.1982.
10
Pyruvate kinase: activation by and catalytic role of the monovalent and divalent cations.丙酮酸激酶：单价和二价阳离子的激活作用及催化作用
Mol Cell Biochem. 1981 Mar 13;35(2):65-75. doi: 10.1007/BF02354821.
本文引用的文献
1
ACCELERATION OF RENAL GLUCONEOGENESIS BY KETONE BODIES AND FATTY ACIDS.酮体和脂肪酸对肾糖异生的促进作用。
Biochem J. 1965 Mar;94(3):712-20. doi: 10.1042/bj0940712.
2
Specificity of pyruvate kinase.丙酮酸激酶的特异性
Biochim Biophys Acta. 1959 May;33(1):238-40. doi: 10.1016/0006-3002(59)90520-7.
3
Adenosine triphosphate conservation in metabolic regulation. Rat liver citrate cleavage enzyme.代谢调节中的三磷酸腺苷守恒。大鼠肝脏柠檬酸裂解酶。
J Biol Chem. 1967 Jul 10;242(13):3239-41.
4
A possible role for acetyl CoA in the control of gluconeogenesis.乙酰辅酶A在糖异生控制中的可能作用。
Adv Enzyme Regul. 1964;2:49-68. doi: 10.1016/s0065-2571(64)80005-4.
5
Citrate and the citrate cycle in the regulation of energy metabolism.柠檬酸与柠檬酸循环在能量代谢调节中的作用
Biochem Soc Symp. 1968;27:23-40.
6
Studies on the glycosidases in jack bean meal. II. Sepation of various glycosidases by isoelectric focusing.
J Biol Chem. 1968 Jul 25;243(14):3994-6.
7
Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: phosphoenolpyruvate carboxylase.棕色固氮菌中磷酸烯醇丙酮酸分支点的调控：磷酸烯醇丙酮酸羧化酶
J Bacteriol. 1971 Apr;106(1):31-6. doi: 10.1128/jb.106.1.31-36.1971.
8
Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. II. Regulation of phosphoenolpyruvate carboxylase and pyruvate kinase.黄色短杆菌中三羧酸循环和乙醛酸循环的调节。II. 磷酸烯醇式丙酮酸羧化酶和丙酮酸激酶的调节
J Biochem. 1969 Sep;66(3):297-311. doi: 10.1093/oxfordjournals.jbchem.a129148.
9
The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.作为调节参数的腺苷酸库的能荷。与反馈调节剂的相互作用。
Biochemistry. 1968 Nov;7(11):4030-4. doi: 10.1021/bi00851a033.
10
Regulation of enzyme function.酶功能的调节
Annu Rev Microbiol. 1969;23:47-68. doi: 10.1146/annurev.mi.23.100169.000403.
Zotero 插件