Ginelli E, Tenchini M L, Di Lernia R
Biochem Exp Biol. 1979;15(3):263-8.
The effect of Phallolysin on cellular growth, macromolecular biosyntheses and cellular membrane structure was analysed using cells from the EUE line. Concentrations of the toxin that do not affect cellular growth, as determined by plating efficiency, have no effect on RNA or protein synthesis, but stimulate DNA synthesis. The doses of Phallolysin that inhibit cell survival do not affect macromolecular biosyntheses, but greatly increase the percentage of cells stainable with Trypan blue after 1 hour of incubation. At the same dose of the toxin the cells, analysed by electron microscopy, show increased vacuolization indicating an alteration of the membrane apparatus.
使用来自EUE系的细胞分析了阴茎溶素对细胞生长、大分子生物合成和细胞膜结构的影响。通过平板接种效率确定的不影响细胞生长的毒素浓度，对RNA或蛋白质合成没有影响，但能刺激DNA合成。抑制细胞存活的阴茎溶素剂量不影响大分子生物合成，但在孵育1小时后会显著增加用台盼蓝染色的细胞百分比。在相同剂量的毒素作用下，通过电子显微镜分析的细胞显示空泡化增加，表明膜细胞器发生了改变。
