Cytotoxic effects of peptichemio on synchronized EUE cells.

The effect of Peptichemio (PTC) on cellular growth and on macromolecular syntheses was analyzed through the cell cycle of EUE cells. The cell response to the various treatments was measured by determining plating efficiency, growth rates and incorporation of labelled precursors of DNA, RNA and protein syntheses. Three maximum inhibition points were found on cell survival, one corresponding to the early G1, another to the middle S and a last one to the late G2. Parallel experiments of incorporation of labelled precursors of DNA, RNA and proteins revealed an effect only on DNA during the early and middle S phase.