正常人肺泡巨噬细胞中苯并[a]芘的代谢模式
Patterns of benzo[alpha]pyrene metabolism in normal human pulmonary alveolar macrophages.
作者信息
Marshall M V, McLemore T L, Martin R R, Jenkins W T, Snodgrass D R, Corson M A, Arnott M S, Wray N P, Griffin A C
出版信息
Cancer Lett. 1979 Dec;8(2):103-9. doi: 10.1016/0304-3835(79)90002-8.
Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[alpha]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[alpha]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[alpha]pyrene metabolites are presented.
从6名正常不吸烟志愿者通过支气管肺泡灌洗获得的肺泡巨噬细胞（PAMs）与[3H] - 苯并[a]芘一起孵育，以确定多环芳烃的正常代谢和结合情况。通过使用粗制葡萄糖醛酸酶制剂，对葡萄糖醛酸和硫酸盐结合物进行了检测。通过高压液相色谱法鉴定出酚类和醌类是与苯并蒽诱导的PAMs孵育1小时期间形成的主要游离代谢产物。此外，酚类和醌类是这些细胞在孵育期间用于结合的主要底物。汇编了非吸烟者的PAMs产生的苯并[a]芘代谢产物范围，并呈现了近致癌物苯并[a]芘代谢产物产生和解毒的变化情况。
Zotero 插件