Nishimura T, Mobley D F, Carlton C E
Urology. 1977 Jan;9(1):39-41. doi: 10.1016/0090-4295(77)90281-3.
As a preliminary investigation for the clinical application of immunoelectrophoresis of ejaculate in the diagnosis of male reproductive organ diseases, variations of immunoelectrophoretic patterns in normal subjects due to the difference of antigenicity between split ejaculates were demonstrated with rabbit antisera. Fresh samples and antisera against them gave larger numbers of and more intense precipitin lines than in stored samples. The precipitin lines developed by acid phosphatase were most remarkable between first portion of ejaculate (F1) and anti-F1, whereas the line caused by protein from the seminal vesicle was most prominent between the remainder of the ejaculate following F1 (F2) and anti-F2.
作为精液免疫电泳在男性生殖器官疾病诊断中临床应用的初步研究，用兔抗血清证明了由于分段精液之间抗原性差异导致的正常受试者免疫电泳图谱的变化。新鲜样本及其相应抗血清产生的沉淀线数量更多、强度更大，比储存样本的情况更明显。酸性磷酸酶产生的沉淀线在射精第一部分（F1）与抗F1之间最为显著，而精囊蛋白产生的沉淀线在F1之后的射精其余部分（F2）与抗F2之间最为突出。
