Scalise M M, Thorp R B, Pretlow T G, Murad T M
Am J Pathol. 1977 Jan;86(1):123-32.
Acinar epithelial cells were purifed from suspensions of cells from the lactating mammary glands of rats. As described previously, this purififaction was accomplished by sedimentation in an isokinetic gradient of Ficoll (polysucrose) in tissue culture medium, and the purity of the separated cells was established by electron microscopy and by histochemical markers. Isoenzymes of glucose-6-phosphate dehydrogenase were investigated at various intervals during lactation in separated populations of stromal and acinar cells. Acinar cells contained three- to eightfold more glucose-6-phosphate dehydrogenase activity than did stromal cells. The proportions of the respective isoenzymes varied during the course of lactation, and the observed changes were parallel in purified acinar cells and in the lactating mammary glands from which the cell suspensions were obtained. The availability of purified acinar cells in the investigation of interactions between hormones and cells from the mammary gland permits a greater degree of specificity than has been possible in the study of mammary cell suspensions which contain myoepithelial cells, duct cells, acinar cells, endothelial cells, fibroblasts, fat cells, mast cells, plasma cells, and blood cells.
从哺乳期大鼠乳腺的细胞悬液中纯化腺泡上皮细胞。如前所述，这种纯化是通过在组织培养基中的等密度Ficoll（聚蔗糖）梯度沉降来完成的，分离细胞的纯度通过电子显微镜和组织化学标记来确定。在哺乳期的不同时间间隔，对分离出的基质细胞和腺泡细胞群体中的葡萄糖-6-磷酸脱氢酶同工酶进行了研究。腺泡细胞中的葡萄糖-6-磷酸脱氢酶活性比基质细胞高3至8倍。在哺乳期过程中，各同工酶的比例有所变化，并且在纯化的腺泡细胞和获得细胞悬液的哺乳期乳腺中观察到的变化是平行的。与含有肌上皮细胞、导管细胞、腺泡细胞、内皮细胞、成纤维细胞、脂肪细胞、肥大细胞、浆细胞和血细胞的乳腺细胞悬液研究相比，纯化的腺泡细胞可用于研究激素与乳腺细胞之间的相互作用，从而具有更高的特异性。
