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薄层色谱法中黄曲霉毒素B1与乙氧喹的无损鉴别

Nondestructive distinction between aflatoxin B1 and ethoxyquin in thin-layer chromatography.

作者信息

Issaq H J, Barr E W, Zielinski W L

出版信息

J Chromatogr. 1977 Feb 1;132(1):115-20. doi: 10.1016/s0021-9673(00)93777-4.

DOI:10.1016/s0021-9673(00)93777-4
PMID:556742
Abstract

A rapid and simple method has been developed for the nondestructive distinction between aflatoxin B1 and the feed antioxidant, ethoxyquin. These two chemicals exhibit similar RF values in certain solvent systems and produce a similar bluish fluorescence under long UV (366 nm) radiation. The method involves the in situ generation of fluorescence spectra of the respective thin-layer chromatography spots. Since it is nondestructive, the method affords ancillary study of the separated aflatoxins.

摘要

已开发出一种快速简便的方法,用于无损区分黄曲霉毒素B1和饲料抗氧化剂乙氧喹。这两种化学物质在某些溶剂系统中表现出相似的比移值,并且在长波紫外线(366纳米)辐射下产生相似的蓝色荧光。该方法涉及原位生成各个薄层色谱斑点的荧光光谱。由于它是无损的,该方法可为分离出的黄曲霉毒素提供辅助研究。

相似文献

1
Nondestructive distinction between aflatoxin B1 and ethoxyquin in thin-layer chromatography.薄层色谱法中黄曲霉毒素B1与乙氧喹的无损鉴别
J Chromatogr. 1977 Feb 1;132(1):115-20. doi: 10.1016/s0021-9673(00)93777-4.
2
Identification of a potential interference in the analysis of aflatoxin, 2,4-dimethyl-6-ethoxyquinoline, a reaction product of ethyoxyquin.鉴定乙氧喹的反应产物黄曲霉毒素、2,4-二甲基-6-乙氧基喹啉分析中的潜在干扰因素。
J Agric Food Chem. 1980 Jan-Feb;28(1):133-5. doi: 10.1021/jf60227a029.
3
Interference of ethoxyquin in the thin-layer chromatographic estimation of aflatoxin.乙氧喹对黄曲霉毒素薄层色谱测定的干扰。
J Chromatogr. 1969 Oct 28;44(2):403-6. doi: 10.1016/s0021-9673(01)92556-7.
4
[Determination of aflatoxin B1 in dried figs by visual screening, thin-layer chromatography and ELISA].[通过目视筛选、薄层色谱法和酶联免疫吸附测定法测定无花果干中的黄曲霉毒素B1]
Z Lebensm Unters Forsch. 1988 Jun;186(6):505-8.
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Thin layer chromatography and densitometric determination of aflatoxins in mixed feeds containing citrus pulp.含柑橘果肉的混合饲料中黄曲霉毒素的薄层色谱法及光密度测定
J Assoc Off Anal Chem. 1986 Jul-Aug;69(4):690-6.
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[Demonstration of aflatoxins in the presence of interfering substances. 2. Studies on the elimination of substances interferring with the TLC determination of aflatoxin B1].[在存在干扰物质的情况下黄曲霉毒素的检测。2. 关于消除干扰黄曲霉毒素B1薄层色谱法测定的物质的研究]
Zentralbl Veterinarmed B. 1976 Apr;23(3):230-42.
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Reverse phase high pressure liquid chromatography and fluorescence detection of ethoxyquin in milk.牛奶中乙氧喹的反相高效液相色谱法及荧光检测
J Assoc Off Anal Chem. 1983 Sep;66(5):1143-7.
8
[Spectrophotometric investigations on aflatoxin B1 and aflatoxin G1 (author's transl)].黄曲霉毒素B1和黄曲霉毒素G1的分光光度研究(作者译)
Arch Geschwulstforsch. 1975;45(1):25-33.
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Rapid thin layer chromatographic method for determining aflatoxin B1, ochratoxin A, and zearalenone in corn.用于测定玉米中黄曲霉毒素B1、赭曲霉毒素A和玉米赤霉烯酮的快速薄层色谱法。
J Assoc Off Anal Chem. 1978 May;61(3):584-5.
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High pressure liquid chromatographic determination of ethoxyquin in paprika and chili powder.高效液相色谱法测定辣椒粉和辣椒末中的乙氧喹啉
J Assoc Off Anal Chem. 1981 Nov;64(6):1453-6.