Issaq H J, Barr E W, Zielinski W L
J Chromatogr. 1977 Feb 1;132(1):115-20. doi: 10.1016/s0021-9673(00)93777-4.
A rapid and simple method has been developed for the nondestructive distinction between aflatoxin B1 and the feed antioxidant, ethoxyquin. These two chemicals exhibit similar RF values in certain solvent systems and produce a similar bluish fluorescence under long UV (366 nm) radiation. The method involves the in situ generation of fluorescence spectra of the respective thin-layer chromatography spots. Since it is nondestructive, the method affords ancillary study of the separated aflatoxins.
已开发出一种快速简便的方法,用于无损区分黄曲霉毒素B1和饲料抗氧化剂乙氧喹。这两种化学物质在某些溶剂系统中表现出相似的比移值,并且在长波紫外线(366纳米)辐射下产生相似的蓝色荧光。该方法涉及原位生成各个薄层色谱斑点的荧光光谱。由于它是无损的,该方法可为分离出的黄曲霉毒素提供辅助研究。