Hembrough F B, Crump M H, Wilke L, Feher R C
Am J Vet Res. 1977 Feb;38(2):245-9.
Purebred 1-year-old Beagles were made hypotensive by controlled hemorrhage (3 ml/kg-1/min-1). Femoral artery blood samples were collected every 5 minutes during controlled hemorrhage, until the blood pressure decreased to 30+/-5 mm Hg. Samples were then collected every 10 minutes for 40 minutes. Blood was not reinfused during the procedure. Carotid blood pressure, electrocardiogram lead II, and respiration rate were recorded. The results of the radioimmunoassay for renin activity indicated that arterial plasma renin activity formed a bimodal activity curve. Plasma renin activity did not reach any plateau, and it continued to increase throughout the experiment. Decreasing blood pressure and heart rate statistically showed a relationship with plasma renin activity (P less than 0.001). There was no interaction between pressure and heart rate; both were strong and independent. Female dogs had a higher plasma renin activity than did male dogs (P less than 0.001) throughout the experimental period. Plasma renin activity remained above resting activity throughout the development of hemorrhagic shock.
将1岁的纯种比格犬通过控制性出血（3毫升/千克/分钟）使其血压降低。在控制性出血期间，每5分钟采集一次股动脉血样，直至血压降至30±5毫米汞柱。然后每10分钟采集一次血样，持续40分钟。在该过程中不进行血液回输。记录颈动脉血压、心电图II导联和呼吸频率。肾素活性放射免疫测定结果表明，动脉血浆肾素活性形成双峰活性曲线。血浆肾素活性未达到任何平台期，且在整个实验过程中持续升高。血压和心率的降低在统计学上与血浆肾素活性相关（P<0.001）。压力和心率之间没有相互作用；两者都很强且相互独立。在整个实验期间，雌性犬的血浆肾素活性高于雄性犬（P<0.001）。在失血性休克发展过程中，血浆肾素活性始终高于静息活性。
