Kolitsky M A, Chervenick P A
J Lab Clin Med. 1977 Apr;89(4):829-35.
In vitro colony-forming cells (CFU-C's) differed from erythrocyte-enhanced colony-forming cells (EE-CFU-C's) in their liquid culture kinetics, adherence properties, and sedimentation velocities. Murine marrow cells stimulated with colony-stimulating activity (CSA) were grown in liquid culture in the presence or absence of washed erythrocytes. A seven to eightfold increase in the number of CFU-C's occurred on day 2 of culture. A somewhat greater increase of nine to 11-fold was observed for EE-CFU-C's which reached a maximum on day 4. When CSA was omitted from the liquid cultures, no increase in CFU-C's or EE-CFU-C's was observed. Marrow cell separation by glass bead adherence columns disclosed a greater degree of adherence for the EE-CFU-C population. Nonadherent cell fractions exhibited a decreased EE-CFU-C/CFU-C ratio (1.19 to 1.24) when compared to controls (3.33) whereas an increase was observed for the eluted, adherent cell population (5.06-6.02). Velocity sedimentation experiments demonstrated a size difference for the marrow-derived CFU-C and EE-CFU-C. CFU-C's sedimented with a velocity of 4.5 mm./hour and EE-CFU-C's sedimented at the rate of 5.7 mm./hour. These observations indicate that EE-CFU-C's appear to represent a population of cells different from the CFU-C's. The later time of peak appearance of EE-CFU-C's in liquid culture suggests that the EE-CFU-C may be a progeny of the CFU-C.
体外集落形成细胞（CFU-C）在液体培养动力学、黏附特性和沉降速度方面与红细胞增强集落形成细胞（EE-CFU-C）有所不同。用集落刺激活性（CSA）刺激的小鼠骨髓细胞在有或没有洗涤过的红细胞存在的情况下进行液体培养。培养第2天，CFU-C的数量增加了7至8倍。EE-CFU-C的数量增加幅度稍大，为9至11倍，在第4天达到最大值。当液体培养中省略CSA时，未观察到CFU-C或EE-CFU-C数量增加。通过玻璃珠黏附柱分离骨髓细胞发现，EE-CFU-C群体的黏附程度更高。与对照组（3.33）相比，非黏附细胞部分的EE-CFU-C/CFU-C比率降低（1.19至1.24），而洗脱的黏附细胞群体的该比率则增加（5.06 - 6.02）。速度沉降实验表明，骨髓来源的CFU-C和EE-CFU-C存在大小差异。CFU-C的沉降速度为4.5毫米/小时，EE-CFU-C的沉降速度为5.7毫米/小时。这些观察结果表明，EE-CFU-C似乎代表了与CFU-C不同的细胞群体。EE-CFU-C在液体培养中出现峰值的时间较晚，这表明EE-CFU-C可能是CFU-C的后代。
