羧甲基纤维素钠和羟乙基淀粉在造血细胞系培养中的作用
Role of sodium carboxymethyl cellulose and hydroxyethyl starch in hematopoietic cell line cultures.
作者信息
Mizrahi A, Moore G E
出版信息
Appl Microbiol. 1971 Apr;21(4):754-7. doi: 10.1128/am.21.4.754-757.1971.
Abstract
Sodium carboxymethyl cellulose and hydroxyethyl starch were found to support rapid growth of two hematopoietic cell lines. The polymers were not metabolized by the cells. In the presence of these compounds, lower rates of glucose utilization and lactic acid production were observed. The uptake of glucose by the cells decreased as the concentration of the polymer in the medium was increased. These results indicate that sodium carboxymethyl cellulose and hydroxyethyl starch probably protect the cells against physical stress in suspended cultures.
摘要
发现羧甲基纤维素钠和羟乙基淀粉能支持两种造血细胞系的快速生长。这些聚合物不会被细胞代谢。在这些化合物存在的情况下，观察到葡萄糖利用率和乳酸生成率降低。随着培养基中聚合物浓度的增加，细胞对葡萄糖的摄取减少。这些结果表明，羧甲基纤维素钠和羟乙基淀粉可能在悬浮培养中保护细胞免受物理应激。
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Partial substitution of serum in hematopoietic cell line media by synthetic polymers.合成聚合物对造血细胞系培养基中血清的部分替代。
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