合成聚合物对造血细胞系培养基中血清的部分替代。
Partial substitution of serum in hematopoietic cell line media by synthetic polymers.
作者信息
Mizrahi A, Moore G E
出版信息
Appl Microbiol. 1970 Jun;19(6):906-10. doi: 10.1128/am.19.6.906-910.1970.
Abstract
Several synthetic polymers (hydroxyethyl starch, sodium carboxymethyl cellulose, polyvinylpyrrolidone) markedly improved the growth of three human lymphocyte cell lines [Roswell Park Memorial Institute (RPMI) 1348, 1788, and 8098]. Growth was stimulated when each of these polymers was added to RPMI 1640 medium supplemented with only 2% fetal bovine serum. Dextran T-40, T-70, and T-110 varied in their effect on the growth of these cell lines. Dextran T-250 and Haemaccel did not improve cell yields when partially substituted for the serum. The successful partial substitution of polymers for serum was specific for individual cell lines.
摘要
几种合成聚合物（羟乙基淀粉、羧甲基纤维素钠、聚乙烯吡咯烷酮）显著促进了三种人淋巴细胞系[罗斯威尔帕克纪念研究所（RPMI）1348、1788和8098]的生长。当将这些聚合物中的每一种添加到仅补充2%胎牛血清的RPMI 1640培养基中时，生长受到刺激。葡聚糖T-40、T-70和T-110对这些细胞系生长的影响各不相同。当部分替代血清时，葡聚糖T-250和贺斯并未提高细胞产量。聚合物对血清的成功部分替代对各个细胞系具有特异性。
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